Sample recording process

  1. Microbes isolation from environmental samples
  2. Environmental (Soil, water)
  3. RNAlater

Culture media

  1. Fungal culture media
  2. Beetle rearing media

Molecular methods

  1. DNA extraction
  2. Soil DNA extraction
  3. PCR w. barcoded primer pairs
  4. Purification (Size selection, 1X ratio)

Nanopore procedures

  1. Library construction (R10 NB barcode) (EvoPrep)
  2. Library construction (R10 PCR barcode) (HyperPrep)
  3. Flow cell wash (PromethION)