Sample recording process

  1. Microbes isolation from environmental samples (soil, water, beetles, and environmental samples.)
  2. Beetles
  3. Fungi
  4. RNAlater

Culture media

  1. Fungal culture media
  2. Beetle rearing media

Molecular methods

  1. DNA extraction
  2. Soil DNA extraction
  3. PCR w. barcoded primer pairs
  4. Electrophoresis
  5. Size selection

Nanopore procedures

  1. Library construction
  2. Sequencing